mouse anti collagen type ii col2 monoclonal antibody Search Results


anti col2  (OriGene)
90
OriGene anti col2
Anti Col2, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/Collagen+II+(COL2A1)+Mouse+Monoclonal+Antibody/pmc04022855-64-35-37
Average 90 stars, based on 1 article reviews
anti col2 - by Bioz Stars, 2026-09
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col2  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology col2
Col2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/COL2A1+Antibody/pm33007484-147-4-5
Average 95 stars, based on 1 article reviews
col2 - by Bioz Stars, 2026-09
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anti col2  (Proteintech)
96
Proteintech anti col2
Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of <t>Col2,</t> ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.
Anti Col2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/MMP13+Antibody/pm39423269-396-18-23
Average 96 stars, based on 1 article reviews
anti col2 - by Bioz Stars, 2026-09
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mouse anti col 2  (Chondrex Inc)
94
Chondrex Inc mouse anti col 2
Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of <t>Col2,</t> ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.
Mouse Anti Col 2, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/Anti-Mouse+Type+I+Collagen+Antibody/pmc03553794-139-15-20
Average 94 stars, based on 1 article reviews
mouse anti col 2 - by Bioz Stars, 2026-09
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rabbit polyclonal anti col 2  (Danaher Inc)
99
Danaher Inc rabbit polyclonal anti col 2
Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of <t>Col2,</t> ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.
Rabbit Polyclonal Anti Col 2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/pmc07240017-81-40-46
Average 99 stars, based on 1 article reviews
rabbit polyclonal anti col 2 - by Bioz Stars, 2026-09
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goat anti type ii collagen  (SouthernBiotech)
92
SouthernBiotech goat anti type ii collagen
Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of <t>Col2,</t> ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.
Goat Anti Type Ii Collagen, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/Goat+Anti-Type+II+Collagen-BIOT/pmc12781688-80-4-10
Average 92 stars, based on 1 article reviews
goat anti type ii collagen - by Bioz Stars, 2026-09
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rabbit type ii collagen col2 polyclonal antibody pa1 26206  (Thermo Fisher)
99
Thermo Fisher rabbit type ii collagen col2 polyclonal antibody pa1 26206
Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of <t>Col2,</t> ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.
Rabbit Type Ii Collagen Col2 Polyclonal Antibody Pa1 26206, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/Collagen/pm39697183-64-14-39
Average 99 stars, based on 1 article reviews
rabbit type ii collagen col2 polyclonal antibody pa1 26206 - by Bioz Stars, 2026-09
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monoclonal mouse anti type ii collagen  (R&D Systems)
93
R&D Systems monoclonal mouse anti type ii collagen
Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of <t>Col2,</t> ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.
Monoclonal Mouse Anti Type Ii Collagen, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/Mouse+Collagen+II+Antibody/10__22203_slash_ecm__v054a01-100-15-30
Average 93 stars, based on 1 article reviews
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mouse anti-col-2 primary antibody (cp18)  (Merck KGaA)
90
Merck KGaA mouse anti-col-2 primary antibody (cp18)
Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of <t>Col2,</t> ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.
Mouse Anti Col 2 Primary Antibody (Cp18), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/mouse+anti+col+2+primary+antibody+cp18/pmc09762312__ADVS___9___2105571___s001-13-0-8
Average 90 stars, based on 1 article reviews
mouse anti-col-2 primary antibody (cp18) - by Bioz Stars, 2026-09
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rabbit anti col2  (Proteintech)
96
Proteintech rabbit anti col2
Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of <t>Col2,</t> ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.
Rabbit Anti Col2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/Aggrecan+Antibody/ppr0631881-64-19-22
Average 96 stars, based on 1 article reviews
rabbit anti col2 - by Bioz Stars, 2026-09
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rabbit anti-mouse col-2  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH rabbit anti-mouse col-2
Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of <t>Col2,</t> ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.
Rabbit Anti Mouse Col 2, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/rabbit+anti+mouse+col+2/pm37169100-111-45-49
Average 90 stars, based on 1 article reviews
rabbit anti-mouse col-2 - by Bioz Stars, 2026-09
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col2 mouse monoclonal antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology col2 mouse monoclonal antibody
( A ) Representative IHC images of <t>COl2,</t> MMP13, and CCDC80 staining in meniscus lesions of the knee joint. ( B ) Quantification of IHC staining intensity for COl2, MMP13, and CCDC80. ( C ) Multivariate analyses of COl2, MMP13 and CCDC80 using GEE to understand the independent therapeutic effect of IOP and hydrogel in the meniscus lesions. All data are shown as means ± standard deviations (n= 6). * P ≤0.05, **** P ≤0.0001. GEE: Generalized Estimating Equation.
Col2 Mouse Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+collagen+type+ii+col2+monoclonal+antibody/normal+mouse+IgG1/bio_rxiv__2025__07__15__663868-62-11-30
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Image Search Results


Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of Col2, ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.

Journal: Science advances

Article Title: Bioenergetic-active exosomes for cartilage regeneration and homeostasis maintenance.

doi: 10.1126/sciadv.adp7872

Figure Lengend Snippet: Fig. 4. Bioenergetic-active exosomes promotes BMSC chondrogenic differentiation and chondrocyte’s homeostasis maintenance through the P2X7-mediated path- way. (A) Hematoxylin and eosin (H&E) (top)–, Toluidine blue (middle)–, Alcian blue (bottom)–stained sections of BMSC microspheres cocultured with N-EXO and Suc-EXO for 28 days. (B) Relative mRNA level of chondrogenic differentiation gene after stimulated by N-EXO and Suc-EXO for 7 days (bottom), 14 days (middle), and 21 days (top) in chondro- genic differentiation medium. (C) Relative mRNA level of Col2, ACAN, MMP9, and MMP13 of BMSCs in common medium. (D) The protein levels of MMP13, MMP9, and Col2 in BMSCs quantified by Western blot. (E) Relative mRNA level of PI3K (top), AKT (middle), and mTOR (down) after incubation with N-EXO, N-EXO, and KN62, Suc-EXO or Suc-EXO and KN62. (F) The protein levels of P2X7, P-mTOR, mTOR, P-S6K, S6K, P-AKT, and AKT in BMSCs quantified by Western blot. (G to I) Relative mRNA level of Col2 (G), ACAN (H), and MMP13 (I) in chondrocytes. (J) The protein levels of MMP13 and Col2 in chondrocytes quantified by Western blot. (K and L) Relative mRNA level of SIRT3 (K) and AMPK (L) in chondrocytes. (M) The protein levels of P2X7, SIRT3, p-AMPK, and AMPK in chondrocytes quantified by Western blot. Results in (B), (C), (E), (G) to (I), and (K) and (L) represent the mean ± SD (n = 3 biological repeats). Statistical analysis: *P < 0.05, **P < 0.01, and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.

Article Snippet: 10, eadp7872 (2024) 18 October 2024 16 of 18 Proteintech, China), anti- MMP13 (1:1000; rabbit polyclonal, Proteintech, China), anti- Col2 (1:1000; rabbit polyclonal, Proteintech, China), anti–phospho- AMPK (1:1000; rabbit polyclonal, Proteintech, China), anti- SIRT3 (1:1000; rabbit polyclonal, ABclonal, China), anti- AMPK (1:1000; rabbit polyclonal, Proteintech, China), anti- P2RX7 (1:1000, rabbit polyclonal, ABclonal, China), anti- AKT(1:1000; rabbit monoclonal, Cell Signaling Technology (CST), USA), anti–phospho- AKT (Ser473) (1:2000; rabbit monoclonal, CST, USA), anti- calnexin (1:1000; rabbit polyclonal, Proteintech, China), anti- S6K (1:2000; rabbit monoclonal, Proteintech, China), anti–phospho- S6K (Thr389) (1:1000; rabbit monoclonal, CST, USA), anti–β- ACTIN (1:20,000; mouse monoclonal, Proteintech, China), and anti- GAPDH (1:50,000, mouse monoclonal, Proteintech, China).

Techniques: Staining, Western Blot, Incubation

Fig. 7. Immunohistochemical and immunofluorescence assessment of cartilage regeneration. (A) Immunohistochemical staining for Col2, ACAN, MMP9, and MMP13. Relative staining intensity of ACAN (B), Col2 (C), MMP9 (D), and MMP13 (E) in cartilage samples at 6 and 12 weeks. (F) Immunofluorescence staining for SIRT3 and Col2 at 12 weeks. Results in (B) to (E) represent the mean ± SD (n = 3 technical repeats). Statistical analysis: **P < 0.01 and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.

Journal: Science advances

Article Title: Bioenergetic-active exosomes for cartilage regeneration and homeostasis maintenance.

doi: 10.1126/sciadv.adp7872

Figure Lengend Snippet: Fig. 7. Immunohistochemical and immunofluorescence assessment of cartilage regeneration. (A) Immunohistochemical staining for Col2, ACAN, MMP9, and MMP13. Relative staining intensity of ACAN (B), Col2 (C), MMP9 (D), and MMP13 (E) in cartilage samples at 6 and 12 weeks. (F) Immunofluorescence staining for SIRT3 and Col2 at 12 weeks. Results in (B) to (E) represent the mean ± SD (n = 3 technical repeats). Statistical analysis: **P < 0.01 and ***P < 0.001, analyzed using ANOVA with Tukey’s multiple comparisons test.

Article Snippet: 10, eadp7872 (2024) 18 October 2024 16 of 18 Proteintech, China), anti- MMP13 (1:1000; rabbit polyclonal, Proteintech, China), anti- Col2 (1:1000; rabbit polyclonal, Proteintech, China), anti–phospho- AMPK (1:1000; rabbit polyclonal, Proteintech, China), anti- SIRT3 (1:1000; rabbit polyclonal, ABclonal, China), anti- AMPK (1:1000; rabbit polyclonal, Proteintech, China), anti- P2RX7 (1:1000, rabbit polyclonal, ABclonal, China), anti- AKT(1:1000; rabbit monoclonal, Cell Signaling Technology (CST), USA), anti–phospho- AKT (Ser473) (1:2000; rabbit monoclonal, CST, USA), anti- calnexin (1:1000; rabbit polyclonal, Proteintech, China), anti- S6K (1:2000; rabbit monoclonal, Proteintech, China), anti–phospho- S6K (Thr389) (1:1000; rabbit monoclonal, CST, USA), anti–β- ACTIN (1:20,000; mouse monoclonal, Proteintech, China), and anti- GAPDH (1:50,000, mouse monoclonal, Proteintech, China).

Techniques: Immunohistochemical staining, Immunofluorescence, Staining

( A ) Representative IHC images of COl2, MMP13, and CCDC80 staining in meniscus lesions of the knee joint. ( B ) Quantification of IHC staining intensity for COl2, MMP13, and CCDC80. ( C ) Multivariate analyses of COl2, MMP13 and CCDC80 using GEE to understand the independent therapeutic effect of IOP and hydrogel in the meniscus lesions. All data are shown as means ± standard deviations (n= 6). * P ≤0.05, **** P ≤0.0001. GEE: Generalized Estimating Equation.

Journal: bioRxiv

Article Title: Evaluating the Therapeutic Efficacy of Iopanoic Acid in a DMM-Induced Osteoarthritis Mouse Model and Osteochondral Lesioned Human Explants

doi: 10.1101/2025.07.15.663868

Figure Lengend Snippet: ( A ) Representative IHC images of COl2, MMP13, and CCDC80 staining in meniscus lesions of the knee joint. ( B ) Quantification of IHC staining intensity for COl2, MMP13, and CCDC80. ( C ) Multivariate analyses of COl2, MMP13 and CCDC80 using GEE to understand the independent therapeutic effect of IOP and hydrogel in the meniscus lesions. All data are shown as means ± standard deviations (n= 6). * P ≤0.05, **** P ≤0.0001. GEE: Generalized Estimating Equation.

Article Snippet: Primary COL2 antibody incubation was done overnight at 4°C with 0.2μg/mL COL2 mouse monoclonal antibody (ab34712, Abcam, Cambridge, MA or with 0.2 μg/mL normal mouse IgG1 as isotype control (sc3877, Santa Cruz, Dallas, TX, USA).

Techniques: Staining, Immunohistochemistry